| Serum-SalivaCorrelation | NA |
| Sensitivity | 5μg/mL |
| SampleTestVolume | 25μL |
| RecommendedCollectionVolume | 75μL* |
| SpecialConsiderations | No |
| CollectionProtocol | DownloadPDF |
Totalproteinisanon-specificmeasureofthetotalamountofallproteinspresentinasolution. Itisusedtoexaminechangesinoverallproteinsecretioninbloodorsalivathatareassociatedwithdiseasestates,ortolookfordifferencesintheratioofspecificproteins(orotheranalytes)tototalproteinthatexistindifferentoralfluidsorthatoccurinresponsetophysiologicalchangesordiseasestates.(1,2,3,4,5,6)TotalproteinisalsosometimesusedtonormalizeconcentrationsofvarioussalivaryproteinssuchasSIgAindifferentsamples,sinceconcentrationscanvarysignificantlyinresponsetostimulationoralterationsofsalivaflow.(5) Ithasbeensuggested,however,thatthispracticemaybemisleADIngbecauseofdifferencesthatexistinthecontrolofsecretionofindividualsalivaryproteinsamongthedifferentsalivaryglands.(7,8,9) Changesintotalproteininsalivaandgingivalcrevicularfluidhavebeenexaminedinrelationtothepresenceofperiodontaldisease,anditmayhavesomeuseasaMarkerofcertaindiseaseconditions.(10,11)
OurlaboratoryusesaPiercebicinchoninicacidassay(BCAassay),whichiscompatIBLewithvariouschemicalsinbuffersthatcancauseinterferenceinotherproteindeterminationmethods,suchastheolderLowrymethod.
- deOliveira,V.N.,Bessa,A.,Lamounier,R.P.,etal.(2010). Changesinthesalivarybiomarkersinducedbyanefforttest. IntJSportsMed,31(6),377-81.
- Aydin,S.(2007).Acomparisonofghrelin,glucose,alpha-amylaseandproteinlevelsinsalivafromdiabetics. JBiochemMolBiol,40(1),29-35.
- Sivakumar,T.,Hand,A.R.,Mednieks,M.(2009). Secretoryproteinsinthesalivaofchildren. JOralSci,51(4),573-80.
- Shi,D.,Meng,H.,Xu,L.,etal.(2008). Systemicinflammationmarkersinpatientswithaggressiveperiodontitis:Apilotstudy. JPeriodontol,79(12),2340-46.
- Lee,J.Y.,Chung,J.W.,Kim,Y.K.,etal.(2007). Comparisonofthecompositionoforalmucosalresidualsalivawithwholesaliva. OralDis,13(6),550-54.
- Furuholm,J.,Sorsa,T.,Qvarnstrom,M.,etal.(2006). Salivarymatrixmetalloproteinase-8inpatientswithandwithoutcoronaryheartdiseasemayindicateanincreasedsusceptibilitytoperiodontaldisease. JPeriodontalRes,41(5),486-89.
- Brandtzeg,P.(2007).Dosalivaryantibodiesreliablyreflectbothmucosalandsystemicimmunity? AnnNYAcadSci,1098,288-311.
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- Bishop,N.C.,Gleeson,M.(2009).Acuteandchroniceffectsofexerciseonmarkersofmucosalimmunity. FrontBiosci,1(14),4444-56.
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- Andersen,E.,Dessaix,I.M.,Mombelli,A.(2010). Myeloid-relatedprotein(MRP8/14)expressioningingivalcrevicefluidinperiodontalhealthanddiseaseandaftertreatment. JPeriodontRes,45(4),458-63.
