| Serum-SalivaCorrelation | 0.91 |
| Sensitivity | <0.007> |
| SampleTestVolume | 25μL |
| RecommendedCollectionVolume | 75μL* |
| SpecialConsiderations | No |
| CollectionProtocol | DownloadPDF |
Cortisol(hydrocortisone,CompoundF)isthemajorglucocorticoidhormoneproducedintheadrenalcortex.Cortisolisactivelyinvolvedintheregulationofcalciumabsorption,bloodpressuremaintenance,anti-inflammatoryfunction,gluconeogenesis,gastricacidandpepsinsecretion,andimmunefunction.(1,2,3)CortisolproductionhasacircADIanrhythm.(4)Levelspeakintheearlymorninganddroptothelowestconcentrationatnight.(5)Levelsriseindependentlyofcircadianrhythminresponsetostress.(6)IncreasedcortisolproductionisassociatedwithCushing’ssyndromeandadrenaltumors,whiledecreasedcortisolproductionisassociatedwithadrenalinsufficiency(e.g.,Addison’sdisease)andadrenocorticotropichormone(ACTH)deficiency.(7)
Inthebloodonly1to15%ofcortisolisinitsunboundorBIOLOGicallyactiveform.Theremainingcortisolisboundtoserumproteins.(8)Unboundserumcortisolentersthesalivaviaintracellularmechanisms,andinsalivathemajorityofcortisolremainsunboundtoprotein.(9)
Salivarycortisollevelsareunaffectedbysalivaryflowrateorsalivaryenzymes.(10)Studiesconsistentlyreporthighcorrelationsbetweenserumandsalivacortisol,indicatingthatsalivarycortisollevelsreliablyestimateserumcortisollevels.(11,12,13)
- Migeon,C.J.,&Lanes,R.L.(1990).Adrenalcortex:hypo-andhyperfunction.InF.Lifshitz(ed.),Pediatricendocrinology,aclinicalguide(2nded.),(pp.333-52).NewYork:MarcelDekker.
- Drucker,S.,New,M.I.(1987). Disordersofadrenalsteroidogenesis.PediatrClinNorthAm,34(4),1055-66.
- Fischbach,F.T.(1992).Themanualoflaboratoryanddiagnostictests,(4thed.).Philadelphia:J.B.Lippincott.
- Dorn,L.D.,Lucke,J.F.,Loucks,T.L.,Berga,S.L.(2007).Salivarycortisolreflectsserumcortisol:analysisofcircadianprofiles.AnnClinBiochem,44(pt3),281-84.
- Chernow,B.,Alexander,H.R.,Smallridge,R.C.,etal.(1987).Hormonalresponsestogradedsurgicalstress.ArchInternMed,147(7),1273-78.
- Kreiger,D.T.(1975).RhythmsofACTHandcorticosteroidsecretioninhealthanddiseaseandtheirexperimentalmodification.JSteroidBiochem,6(5),758-91.
- Rothfield,B.(1974).Plasmacortisol.In:B.Rothfield(ed.),Nuclearmedicine–invitro(pp.120-5).Philadelphia:Lippincott.
- Robin,P.,Predine,J.,Milgrom,E.(1977).Assayofunboundcortisolinplasma.JClinEndocrinolMetab,46(2),277-83.
- Vining,R.F.,McGinley,R.A.,Symons,R.G.(1983).Hormonesinsaliva:modeofentryandconsequentimplicationsforclinicalinterpretation.ClinChem,29(10),1752-56.
- Vining,R.F.,McGinley,R.A.(1987).Themeasurementofhormonesinsaliva:Possibilitiesandpitfalls.JSteroidBiochem,27(1-3),81-94.
- Francis,S.J.,Walker,R.F.,Riad-Fahmy,D.,etal.(1987).Assessmentofadrenocorticalactivityintermnewborninfantsusingsalivarycortisoldeterminations.JofPediatrics,111,129-33.
- Hiramatsu,R.(1981).Directassayofcortisolinhumansalivabysolidphaseradioimmunoassayanditsclinicalapplications.ClinicaChimicaActa,117,239-249.
- Vining,R.F.,McGinley,R.A.,Maksvytis,J.J.,Ho,K.Y.(1983).Salivarycortisol:Abettermeasureofadrenalcorticalfunctionthanserumcortisol.AnnClinBiochem,20(pt6),329-35.
